Nanocellulose and Cellulose Making with Bio-Enzymes from Different Particle Sizes of Neosinocalamus Affinis

Cellulose is one of the most abundant, widely distributed and abundant polysaccharides on earth, valuable natural renewable resource for human beings. In this study, three different particle sizes (250, 178, 150 ?m) Neosinocalamus affinis cellulose were extracted from powder using bio-enzyme digestion prepared into nanocellulose (CNMs). The contents 250, ?m 53.44%, 63.38%, 74.08%, respectively; crystallinity was 54.21%, 56.03% 63.58%, respectively. thermal stability increased gradually with smaller sizes. yields CNMs 14.27%, 15.44%, 16.38%, results showed that successfully removed lignin, hemicellulose, impurities (pectin, resin, etc.) by treatment A (ligninase:hemicellulose:pectinase = 1:1:1) combined NH3·H2O H2O2/CH3COOH. Extraction A, size powder, more content extracted, higher crystallinity, better stability, purity. Subsequently, (CNMs) B (cellulase:pectinase 1:1). a network structure fibrous bundle shape. Therefore, ones in study belong to nanofibrils (CNFs). This provides reference extraction bamboo bio-enzymes preparation nanocellulose. To certain extent, utilization as biomass material improved.